Agilent Fiber Optic Sensing enables remote, in situ measurements using fiber optic probes integrated with UV-Vis and fluorescence spectrophotometers for versatile, accurate, and non-invasive analysis.

Overview of Agilent Fiber Optic Sensing

Agilent's fiber optic sensing technology transforms standard UV-Vis and fluorescence spectrophotometers into remote measurement systems, allowing users to analyze samples that are difficult to access, sensitive, hazardous, or too large for conventional instruments . This is achieved through fiber optic couplers, cables, and specialized probes, which connect to the spectrophotometer and transmit light to and from the sample.

How Fiber Optic Probes Work

Fiber optic probes are designed to measure liquids, solids, and surfaces without transferring samples to cuvettes, reducing sample loss and user error . Key features include:

• Probe tips: Available in stainless steel, Torlon, or quartz, with reusable or disposable options.
• Path lengths: Standard and microprobes for low-volume measurements.
• Reflection/transmission probes: For solid surface analysis, including fiber optic reflectance probes (remote DRA).
• Ambient light immunity: Allows measurements in bright environments, including outdoors. These probes can be used in glove boxes, cold storage, or production lines, enabling real-time monitoring and efficient workflow integration.

Applications

Agilent fiber optic sensing is ideal for:

• In situ reaction monitoring in chemical or biological experiments.
• Air-sensitive or hazardous samples, where direct handling is risky.
• Large or irregularly shaped samples that cannot fit in standard cuvettes.
• Production line monitoring, improving efficiency and reducing downtime. The technology supports a wide range of sample volumes, from microliters to large-scale samples, and ensures reliable, reproducible results by minimizing common issues like tubing leaks, bubbles, or condensation .

Compatible Instruments

Agilent's Cary 60 UV-Vis and Cary Eclipse fluorescence spectrophotometers are optimized for fiber optic measurements, offering:

• Highly focused beams for efficient fiber coupling.
• Sufficient photodynamic range for accurate spectroscopy.
• Ease of integration with fiber optic accessories for remote sensing .

Advantages

• Non-invasive and fast measurements without sample transfer.
• Versatile probe options for liquids, solids, and surfaces.
• Enhanced safety for hazardous or sensitive samples.
• Operational flexibility in various environments, including production lines and glove boxes.
• Reduced maintenance and cleaning compared to traditional cuvettes. Agilent fiber optic sensing provides a powerful solution for laboratories and industrial applications, enabling precise, remote, and efficient spectroscopic measurements that expand the capabilities of standard UV-Vis and fluorescence instruments .